Conventional agriculture systems of production often lead to environmental degradation, economic problems and even social conflict. The efficacy of agriculture systems conducive to the economic, environmental and social sustainability of farming operations has been demonstrated, yet the adoption of sustainable agricultural practices is not widespread. This study evaluates the barriers of sustainable agriculture by wheat farmers in Takestan using a descriptive–correlation survey methodology. This quantitative study was based on a Researcher made questionnaire designed to elicit the barriers to adoption of sustainable agriculture practices perceived by a sample of 149 wheat farmers in the Takestan (N=268), through a stratified random sampling technique. Instrument validity was confirmed by a panel of experts. The reliability estimated by Cronbach’s coefficient (α=0.905). The data has been analyzed using the SPSS (16). The results revealed that the high cost of sustainable agriculture was the most important barriers in its implementation (M=4.74). The findings of multiple regressions explained that farmer’s attitudes and practices of sustainable agriculture explained 89 percent of the variance of the barriers of sustainable agriculture. Farmer’s attitudes had the most influence on the determination of the barriers of sustainable agriculture (β=0.775).