Sustainable agricultural and rural development requires knowledge and information, skills, attitudes and technologies, which run through a network of actors to produce, distribute and use it in a particular place. The model of the Agriculture Knowledge and Information Systems (AKIS) is designed based on this recognition. In Iran, There are about decades of experience in the development of this system. The purpose of this Delphi study was to develop a listing of those challenges facing Iran's Agricultural Knowledge and Information System (AKIS). Panel of experts consisted of extension offices, natural-level researchers, faculty members of Agricultural Research, Education and Extension Organization (AREEO), and university faculty in agricultural extension and education. The panel identified 86 challenges of AKIS in Iran that were classified into 8 categories consisting of Participatory-institutional, Policy-legal, Information broadcasting-communicational, Monitoring and evaluation, Actors competency, supportive-motivational, management-structural and infrastructure-environmental.